Blog

One College Avenue DIF Williamsport PA Tel Toll Free Go Bing homepageSign My saves Manufacturers Centerimcpa View All Through the assistance of we have been able develop quality high temperature grout well superior Oil Field Containment System both which opened new doors and possibilities into growing industry for Polymics. Unless there a different instruction from antibody manufacturer minor changes can be made according to applications such as fluorescent IHC rule is use milk for Western Blots and BSA . So you would get higher background with BSA blocking | Selectively transform lignin into value-added chemicals ...

Read more years UniversityWe routinely use skimmed milk TBST for Westerns. I dont think TBST or PBST make huge difference. leh function r

In this case you might think to block with milk which has stronger blocking ability Read more year agoIbrahim Hamza KankiaUmaru Musa Yar adua University KatsinaAlthough agree that casein the can interfere some cases phosphoproteins also works fine and manufacturers normally recommend using instead of BSA. P var d appendChild g createElement src k div f iframe document yle. display block return if function yle. test true l d f try ntentWindow catch main p javascript var void . Signal amplification was performed using the appropriate ABC kit Vector Laboratories and immunocomplexes were detected with DAB staining. Read more years agoSusana de Investigaciones del Hombre en DesiertoI don work with phosphorylated proteins but saw milk TBSt leave the Western blots high background all black

I have been already writing to you about this method. Hope this helps Chris Read more years agoGabriela of South AlabamaFor phosphoproteins use always BSA. For some extremely temperamental antibodies ve had luck using non protein blocking agents. hbncl sj evt re BNCHCLoaded w var Notifications u ApiFailure y Latency bnchide bnchca qs ge chcc sb gt evtClick BncHeadCtrl sch function nd onP setTimeout SearchBox header be dy form cook CW window UTC new Date String null SRCHHPGUSR resize . If you get high background suugest to increase the number of washing steps with PBST. All rights orDiscover by subject areaJoin for freeLog Skip to main content Please note that Internet Explorer version. Its expensive but you will get very clean blots with all the antibodies. IG u s o navigator fd ls lsp px else sj log function return setHeight for . on behalf of Chinese Chemical Society and Institute Materia Medica Academy Medical Sciences. I know about the phosphocasein milk but don mouse monoclonal. Most of ur buffers is based on tris and using buffered saline with tween works well then phosphate . Read more you help by adding an your answerSimilar questions and discussionsWhat the difference between nonfat milk BSA for primary antibody western blotSome protocols mention antibodies should diluted whereas