Bpbovine growth hormone polyadenylation signal bGH . ew ScholarNelson CE Hakim Ousterout DG Thakore PI Moreb EA Castellanos Rivera RM et al. a and Additional file Figure Sa KP carries nt CG deletion in the GD allele otherwise wildtype WT Kras GG [...]
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Novel splice isoforms could encode proteins that retain partial function and should be carefully considered when interpreting phenotypes result from CRISPRinduced mutations frequency with which indels cause exon skipping difficult to predict. bppackaging signal of human virus type SV poly [...]
A cells were transduced with sgCtnnb. sgRNAs that use NGG PAM are shown above exon and those CCN below . We isolated two singlecell clones each carrying frameshifting deletions exon Fig [...]
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In cells transduced with Dmdsg we also detected RTPCR product corresponding to exon spliced Fig. Skipping of exon will generate inframe mRNA. It opens many common file formats. Indels of sg were not determined [...]
Multiplex genome engineering using CRISPR Cas systems. The other two products correspond to alternatively spliced transcripts one that skips exon . e ew ScholarYin Xue Chen Bogorad RL Benedetti Grompe al [...]
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Bpcore promoter for human elongation factor EF CMV . answers addedHow do I calculate the annealing temperature of my PCR reactionTm value one primer is [...]
PDF kb Additional file Supplemental Table. was used to identify insertions and deletions with the pileupindel mode parameters minvar freq minavg qual pvalue. ew ScholarTuveson DA Shaw AT Willis NA Silver DP Jackson EL Chang et al. kDa Try SnapGene and create your own beautiful maps Individual Sequences Viewer offers the fastest easiest way to plan visualize document molecular biology procedures [...]
