M Sodium phosphate buffer . unbind opfOpenEnd w sj evt re opfOpenStart else function be var et chromewebstore item chromeinline extn ef ft ot ge opalpers anch flyout onP appHTML if ildNodes moveChild for CI yleExp sendBeacon navigator fd lsp px log return setHeight . Craig S Pikaard in Methods Enzymology Solutions Needed for Xenopus saline PBS mM NaCl NaHPO [...]

With Mg Ca PBS Sigma Magnesium chloride MgCl Calcium CaCl Sodium dodecyl sulfate SDS Tris acid HCl hydroxide NaOH EDTA Dithiothreitol DTT NaCl Nonidet NP deoxycholate DOC acetate NaOAc complexes VRC New England Biolabs fluoride PMSF Torula yeast RNA cRNA globin alternative control AntiFLAG affinity gel Proteinase KGlycoblue Ambion Phenol chloroform isoamyl alcohol ethanolRead full chapterView topic accessShopping cartContact supportTerms policyWe use cookies help provide enhance our service tailor content ads. Dent s fixative methanol DMSOBT formaldehyde from . This procedure detailed in the section handling embryos Ref. Bedford MA ChloramineT CT mg ml mM sodium phosphate buffer and made up within of use is light sensitive the solution amber vialSodium metabisulfite fresh day iodination this reagent moisture care taken to keep stock proGnRH [...]

StemCell Technologies Preparation of complete NSC medium is thoroughly described technical manuals asp with the inlaboratory components next section combining ml NeuroCult basal proliferation supplement will produce growth Stock Solutions item . TweenBlocking solution PBS with DMSO . StemCell Technologies hormone mix NeuroCult NSC proliferation supplement cat [...]

This procedure detailed in the section handling embryos Ref. with Mg Ca PBS Sigma Magnesium chloride MgCl Calcium CaCl Sodium dodecyl sulfate SDS Tris acid HCl hydroxide NaOH EDTA Dithiothreitol DTT NaCl Nonidet NP deoxycholate DOC acetate NaOAc complexes VRC New England Biolabs fluoride PMSF Torula yeast RNA cRNA globin alternative control AntiFLAG affinity gel Proteinase KGlycoblue Ambion Phenol chloroform isoamyl alcohol ethanolRead full chapterView topic accessShopping cartContact supportTerms policyWe use cookies help provide enhance our service tailor content ads [...]

Please refer to this blog post for more information. in Ref. Sucrose [...]

Le Feedback t handler failed in . BSA dissolve the by heating solution to C. M glutamate in NaOH . StemCell Technologies Differentiation medium NeuroCult supplement cat [...]

StemCell Technologies hormone mix NeuroCult NSC proliferation supplement cat. Recipes for egg collection plates can be found in the references mentioned above [...]

This procedure detailed in the section handling embryos Ref. M NaHPO . Tween Normal Goat SerumHighsalt wash PBS with DMSO . Abrams in Methods Enzymology General buffer pH [...]

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Conrad in Methods Enzymology Saline pH. Tween Normal Goat Serum. To obtain large batches of staged embryos frequent replacement these plates is advised [...]

StemCell Technologies Basal medium NeuroCult NSC cat. end u. V LX Group. mM EGTA DTT [...]